The formulation of LowCross-Buffer® helps to reduce interference in immunoassays. Thus quality of detection can be significantly improved. Nonspecific binding of the antibodies, negative effects of interfering substances and low or medium affinity cross-reactivities of the antibodies are minimized. In addition matrix effects, coming for example from blood sera or plasma specimen are reduced. That means that matrix effects no longer exacerbate detection of analytes. Minimization of all these negative effects upgrades the quality of the assay and improves the reliability of the results.